sticky-end ligation

См. также в других словарях:

• Trans-Spliced Exon Coupled RNA End Determination (TEC-RED) — Trans Spliced Exon Coupled RNA End Determination is a technique designed by Muller et al. that, like SAGE, allows for the digital detection of messenger RNA sequences. Unlike SAGE, detection and purification of transcripts from the 5’ end of the… …   Wikipedia

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• TA cloning — Cloning strategy for PCR products that relies on the tendency of Taq polymerase to add an extra dA at the 3&’ end of newly synthesized DNA strands, thus leaving a single base 3&’ overhang. Vectors are accordingly prepared with single base dT 3&’… …   Dictionary of molecular biology